Genome Of Rifamycin B ProducingAmycolatopsis Mediterranei S699 (Accession No. CP002896)

Whole genome sequencing of strain S699 was performed by using Roche 454 system (GS20 version) and Sanger shotgun sequencing. The hybrid assembly of Sanger-pyrosequencing data using Phrap resulted in 386 contigs. Subsequently, sequence gaps were filled by primer walking, transposon mutagenesis and complete sequencing of linker clones using Roche 454 system (GS FLX). The final assembly that consisted of 1,993,024 reads was done with MIRA3 Assembler by mapping to the reference genome of A. mediterranei U32 (GenBank accession number CP002000). The complete genome sequence of A. mediterranei S699 contains a single circular 10,236,779 bp chromosome with a GC content of 71.3 %. There are 9,575 CDSs falling into 6,883 functional COG groups. For details please see:

Important Papers

• Verma, M., Kaur, J., Kumar, M., Kumari, K., Saxena, A., Anand, S., Nigam, A., Ravi, V., Raghuvanshi, S., Khurana, P., Tyagi, A.K., Khurana, J.P., and Lal, R . (2011) Whole Genome Sequence of Rifamycin B Producing Amycolatopsis mediterranei S699. J. Bacteriol. 193: 5562-5563.

Genome Of Hexachlorocyclohexane (HCH) Degrading Bacterium Sphingobium Indicum B90A (Accession No. AJXQ00000000)

Sphingobium indicum B90A was isolated from sugarcane rhizosphere soil in 1990 and became the first strain to degrade one of the most recalcitrant man-made compounds, the β- hexachlorocyclohexane (HCH). Since then B90A has been the focus of many studies leading to novel discoveries such as the association of lin genes with IS 6100 , enantioselective transformation of chiral α-HCH by linA1 and linA2 gene, the presence of efficient HCH dehydrochlorinase and haloalkane dehalogenase systems, evolution of isomeric specific (α-, β-, γ-, δ- and ε- HCH) degradation pathways and potential application of B90A in HCH bioremediation. The draft genome of S. indicum B90A was generated by using Illumina Genome Analyzer platform. The sequencing data (88.3% of the total raw reads) was assembled into 149 contigs
The availability of the genome sequence of S. indicum B90A coupled with community genomics data from the HCH dumpsite (R. Lal-unpublished) will act as an invaluable supplement to the ongoing research efforts towards understanding several unanswered questions associated with the degradation of HCH isomers and would thus aid in the development of in situ ; bioremediation technology in the future. For details please see:

Important Papers

• Anand, S., Sangwan, N., Lata, P., Kaur, J., Dua, A., Singh, A., Verma, M., Kaur J., Khurana, P., Khurana, J.P., Mathur, S., and Lal, R. 2012. Genome Sequence of Sphingobium indicum B90A, a Hexachlorocyclohexane (HCH) Degrading Bacterium. J. Bacteriol . 194:4472.

Genome Of Thermus Sp. RLT ( Accession No. AIJQ00000000)

Thermus sp. RL genome featured on the cover page of J.Bacteriology 194,3534 (cover page can be viewed at http://jb.asm.org/content/194/13.cover-expansion)

The hot water springs located atop the Himalayan ranges in Parvati Valley at an altitude of 1,760 m in Manikaran, India, are known for their scenic beauty and spiritual sanctity. These are the hottest springs (90°C to 98°C) in the country and contain low levels of helium. The microbial diversity from these hot water springs has not been explored previously. Thermus sp. strain RL was isolated from a hot water spring, and its genome was sequenced. Metagenomic analysis is being carried out to understand microbial diversity at this site and to understand microbial life and biochemical processes at these high temperatures
Thermus sp. RL represents genome size of 20,36,600 bp with an average G+C content of 68.77%. Genome annotations predicted 1986 protein coding genes and 710 hypothetical proteins. Strain RL has two rRNA operons (5S-16S-23S and 5S-5S-16S-23S ) and 47 tRNA genes . A total of 111 tandem repeats, 2825 CpG islands and a single CRISPR element.Further determination of the taxonomic position of strain RL, comparative genomics of Thermus spp. and metagenomic analyses of the hotspring are under way. We are also investigating the Taq DNA polymerase activity of this stain.For details please see

Important Papers

• Dwivedi, V., Sangwan, N., Nigam, A., Garg, N., Niharika, N., Khurana, P., Khurana, J.P., and Lal, R. Draft Genome Sequence of Thermus sp. RL Isolated from Hot Water Spring Located atop the Himalayan Ranges at Manikaran, India . J. Bacteriol. 194:3534.

Genome Of Acinetobacter Sp. HA (Accession No. AJXD00000000)

Acinetobacter sp. HA was isolated from the gut of the insect Helicoverpa armigera . It has a G+C content of 41% and the estimated genome size is 3.12Mb that has been assembled into 102 contigs. The bacterium possesses genes for natural competence as well as for esterase activity. The genome is being proceeded with for gap filling at the finishing stage by sequencing of a cosmid library (1156 clones in Super cos vector) that will validate the overall assembly. For details please see
Malhotra, J., Dua, A., Saxena, A., Sangwan, N., Mukherjee, U., Pandey, N., Raman, R., Khurana, P., Khurana, J,P and Lal, R. Genome sequence of Acinetobacter sp. HA isolated from the gut of polyphagous insect pest Helicoverpa armigera. J. Bacteriol. 194: 5156

More Genome Announcements:

• Niharika, N., Sangwan, N., Ahmad, S., Singh, P., Khurana, J.P. and Lal, R. 2013. Draft genome sequence of Sphingobium chinhatense strain IP26 T isolated from the hexachlorocyclohexane dumpsite. Genome Announc . doi:10.1128/genomeA.00680-13.
• Saxena, A., Nayyar, N., Sangwan, N., Kumari, R., Khurana, J.P., and Lal, R. 2013. Genome Sequence of Novosphingobium lindaniclasticum LE124 T , isolated from a Hexachlorocyclohexane (HCH) dumpsite. Genome Announc . doi:10.1128/genomeA.00715-13.
• Singh, A. K., Sangwan, N., Sharma, A., Gupta, V., Khurana, J.P., and Lal, R . 2013. Draft Genome Sequence of Sphingobium quisquiliarum P25 T , a Novel Hexachlorocylohexane (HCH) Degrading Bacterium Isolated from the HCH Dumpsite. Genome Announc doi:10.1128/genomeA.00717-13.
• Mukherjee, U., Kumar, R., Mahato, N.K.., Khurana, J.P., and Lal, R. 2013. Draft Genome Sequence of Sphingobium sp. HDIPO4, an Avid Degrader ofHexachlorocyclohexane. Genome Announc. doi:10.1128/genomeA.00749-13.
• Kaur, J., Verma, H., Tripathi, C., Khurana, J.P., and Lal, R. 2013. Draft Genome Sequence of a Hexachlorocyclohexane-DegradingBacterium, Sphingobium baderi Strain LL03 T . Genome Announc. doi:10.1128/genomeA.00751-13.
• Kumar, R., Dwivedi, V., Negi, V., Khurana, J.P., and Lal, R. 2013. Draft Genome Sequence of Sphingobium lactosutens DS20Hexachlorocyclohexane (HCH) Dumpsite. Genome Announc. doi:10.1128/genomeA.00753-13.
• Mahato NK., Tripathi C., Verma H., Singh N., Lal R. 2014. Draft genome sequence of Deinococcus sp. strain RL isolated from sediments of hot Spring located at Manikaran, India. Genome Announc. doi:10.1128/genomeA.00703-14.
• Mukherjee U., Saxena A., Kumari R., Singh P., Lal R. 2014. Draft genome sequence of Amycolatopsis mediterranei DSM 40773, a tangible antibiotic producer. Genome Announc. doi: 10.1128/genomeA.00752-14.
• Saxena A., Kumari R., Mukherjee U., Singh P., Lal R . 2014. Draft Genome Sequence of Rifamycin producer Amycolatopsis rifamycinica 46095. Genome Announc. doi:10.1128/genomeA.00662-14.
• Negi V., Lata Pushp, Sangwan N., Gupta S., Das S., Rao D.L.N., and Lal R . Draft genome sequence of Hexachlorocyclohexane (HCH)- degrading Sphingobium lucknowense Strain F2, isolated from the HCH Dumpsite. Genome Announc. doi:10.1128/genomeA.00788-14.
• Singh P., Kumari R., Mukherjee U., Saxena A., Sood U., Lal R. Draft genome sequence of Rifamycin derivatives producing Amycolatopsis mediterranei strain DSM 46096/S955. Genome Announc. doi:10.1128/genomeA.00837-14.
• Sharma A, Hira P, Shakarad M, Lal R . 2014. Draft genome sequence of Cellulosimicrobium sp. MM, isolated from arsenic rich microbial mats of a Himalayan Hot Spring. Genome Announc . 5: e01020-14.