Prof. Rup Lal

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HCH Bioremediation

Another major area his group is working on, is the molecular genetics of hexachlorocyclohexane (HCH) degradation by sphingomonads that could eventually lead to the development of a technology for bioremediation of HCH contaminated soils. He has extensively studied the degradation of HCH by Sphingobium indicum (formerly known as Sphingomonas paucimobilis B90A). His group has unravelled the physiological and genetic aspects of HCH degradation in B90A and has provided evidence for the stability horizontal transfer of ' lin ' genes (responsible for HCH degradation) among different bacteria. The study has also led to the cloning of genes responsible for the mineralization of α- and γ- isomers of HCH in B90A and dynamics of ' lin ' gene expression. The group is working on deciphering the degradation pathway (hitherto not known) of α- δ- β and ε- HCH. Alongside, his group is attempting bioremediation of HCH contaminated sites by 'bioaugmentation', 'biostimulation' and 'enzymatic bioremediation' approaches..

 

Important Papers

  • Phian, S. Verma, H Singh, DN. Singh, Y. Lal, R. and Dogra C. 2024. Comparative genomics reveal unique markers to monitor by routine PCR assay bioinoculant of Sphingobium indicum B90A in hexachlorocyclohexane (HCH) contaminated soils.Indian J Microbiol. 64(3), 1266–1277.https://doi.org/10.1007/s12088-024-01321-7
  • Sharma, M., Singh, D.N., Budhraja, R., Sood, U., Rawat, C.D., Adrian, L., Richnow,H.H., Singh, Y., Negi, R.K. and Lal, R., 2021. Comparative proteomics unravelled the hexachlorocyclohexane (HCH) isomers specific responses in an archetypical HCH degrading bacterium Sphingobium indicum B90A.Environ. Sci. Pollut. Res. 28: 41380-41395
  • Singh, P., Kumari, R., Nayyar, N., and Lal, R. 2017. Pontibacter aurantiacus sp. nov. isolated from hexachlorocyclohexane (HCH) contaminated soil. Int. J. Syst. Evol. Microbiol. 67: 1400-1407.
  • Garg, N., Lata, P., Jit, S., Sangwan, N., Singh, A. K., Dwivedi, V., Niharika, N., Kaur, J., Saxena, A., Dua, A., Nayyar, N., Kohli, P., Gueke, B., Kunz, P., Rentcsch, D., Holliger, C., Kohler, H. P., Lal, R. 2016. Laboratory and field scale bioremediation of hexachlorocyclohexane (HCH) contaminated soils by means of bioaugmentation and biostimulation. Biodegradation. 27: 179-193.
  • Verma, H., Rani, P., Singh, A. K., Kumar, R., Dwivedi, V., Negi, V., Lal, R. 2015. Sphingopyxis flava sp. nov., isolated from an hexachlorocyclohexane (HCH)-contaminated soil, India. Int. J. Syst. Evol. Microbiol. 65: 3720-3726.
  • Verma, H., Rani, P., Singh, A. K., Kumar, R., Dwivedi, V., Negi, V., Lal, R. 2015. Sphingopyxis flava sp. nov., isolated from an hexachlorocyclohexane (HCH)-contaminated soil, India. Int. J. Syst. Evol. Microbiol. 65: 3720-3726.
  • Kumar, R., Dwivedi, V., Nayyar, N., Verma, H., Singh, A. K., Rani, P., Rao, D. L. N., Lal, R. 2015. Parapedobacter indicus sp. nov., isolated from hexachlorocyclohexane (HCH) contaminated soil. Int. J. Syst. Evol. Microbiol. 65: 129-134.
  • Verma, H., Kumar, R., Oldach, P., Sangwan, N., Khurana, J. P., Gilbert, J. A., Lal, R. 2014. Comparative genomic analysis of nine Sphingobium strains: Insights into their evolution and hexachlorocyclohexane (HCH) degradation pathway. BMC Genomics 15: 1014.
  • Negi, V., Lata, P., Sangwan, N., Gupta, S. K., Das, S., Rao, D. L. N., Lal, R. 2014. Draft genome sequence of hexachlorocyclohexane (HCH) degrading Sphingobium lucknowense strain F2T isolated from the HCH dumpsite. Genome Announc. 2: e00788-14.
  • Singh, A. K., Garg, N., Lata, P., Kumar, R., Negi, V., Vikram, S., Lal, R. 2014. Pontibacter indicus sp. nov., isolated from hexachlorocyclohexane (HCH) contaminated soil. Int. J. Syst. Evol. Microbiol. 64: 254-259.
  • Dua, A., Malhotra, J., Saxena, A., Khan, F., Lal, R. 2013. Devosia lucknowensis sp. nov., a bacterium isolated from hexachlorocyclohexane (HCH) contaminated pond soil. J. Microbiol. 51: 689-694.
  • Gupta, S. K., Lal, D., Lata, P., Sangwan, N., Garg, N., Holliger, C., Lal, R. 2013. Changes in the bacterial community and lin genes diversity during biostimulation of indigenous bacterial community of hexachlorocyclohexane (HCH) dumpsite soil. Microbiol. 82: 234-240.
  • Jindal, S., Dua, A., Lal, R. 2013. Sphingopyxis indica sp. nov., isolated from a high dose point hexachlorocyclohexane (HCH) contaminated dumpsite. Int. J. Syst. Evol. Microbiol. 63: 2186-2191.
  • Saxena, A., Nayyar, N., Sangwan, N., Kumari, R., Khurana, J. P., Lal, R. 2013. Genome Sequence of Novosphingobium lindaniclasticum LE124T, isolated from a hexachlorocyclohexane (HCH) dumpsite. Genome Announc. 1: e00715-13.
  • Singh, A. K., Sangwan, N., Sharma, A., Gupta, V., Khurana, J. P., Lal, R. 2013. Draft genome sequence of Sphingobium quisquiliarum P25T, a novel hexachlorocyclohexane (HCH)-degrading bacterium isolated from the HCH dumpsite. Genome Announc. 1: e00717-13.
  • Malhotra, J., Anand, S., Jindal, S., Rajagopal, R., Lal, R. 2012. Acinetobacter indicus sp. nov., isolated from hexachlorocyclohexane (HCH) dumpsite. Int. J. Syst. Evol. Microbiol. 62: 2883-2890.
  • Niharika, N., Jindal, S., Kaur, J., Lal, R. 2012. Sphingomonas indica sp. nov., isolated from hexachlorocyclohexane (HCH)-contaminated soil. Int. J. Syst. Evol. Microbiol. 62: 2997-3002.
  • Lal, D., Khan, F., Gupta, S. K., Schumann, P., Lal, R. 2012. Edaphobacillus lindanitolerans gen. nov., sp. nov., isolated from hexachlorocyclohexane (HCH) contaminated soil. J. Basic Microbiol. 53: 758-765.
  • Geueke, B., Garg, N., Ghosh, S., Fleischmann, T., Holliger, C., Lal, R., Kohler, H. P.-E. 2012. Metabolomics of HCH-transformation: LinA to LinB ratio determines metabolic fate of HCH isomers. Environ. Microbiol. 15: 1040-1049.
  • Saxena, A., Anand, S., Dua, A., Sangwan, N., Khan, F., Lal, R. 2012. Novosphingobium lindaniclasticum sp. nov., a hexachlorocyclohexane (HCH)-degrading bacterium isolated from HCH dumpsite. Int. J. Syst. Evol. Microbiol. 63: 2160-2167.
  • Garg, N., Bala, K., Lal, R. 2011. Sphingobium lucknowense sp. nov., a hexachlorocyclohexane (HCH)-degrading bacterium isolated from HCH-contaminated soil. Int. J. Syst. Evol. Microbiol. 62: 618-623.
  • Vijgen, J., Abhilash, P. C., Li, Y. F., Lal, R., Forter, M., Torres, J., Singh, N., Yunus, M., Tian, C., Schäffer, A., Weber, R. 2010. Hexachlorocyclohexane (HCH) as new Stockholm Convention POPs — a global perspective on the management of Lindane and its waste isomers. Environ. Sci. Pollut. Res. Int. 18: 152-162.
  • Bala, K., Sharma, P., Lal, R. 2010. Sphingobium quisquiliarum sp. nov., a hexachlorocyclohexane (HCH)-degrading bacterium isolated from an HCH-contaminated soil. Int. J. Syst. Evol. Microbiol. 60: 429-433.
  • Dadhwal, M., Jit, S., Kumari, H., Lal, R. 2009. Sphingobium chinhatense sp. nov., a hexachlorocyclohexane (HCH)-degrading bacterium isolated from an HCH dumpsite. Int. J. Syst. Evol. Microbiol. 59: 3140-3144.
  • Verma, M., Kumar, M., Dadhwal, M., Kaur, J., Lal, R. 2009. Devosia albogilva sp. nov. and Devosia crocina sp. nov., isolated from a hexachlorocyclohexane (HCH) dump site. Int. J. Syst. Evol. Microbiol. 59: 795-799.
  • Dadhwal, M., Singh, A., Prakash, O., Gupta, S. K., Kumari, K., Sharma, P., Jit, S., Verma, M., Holliger, C., Lal, R. 2009. Proposal of biostimulation for hexachlorocyclohexane (HCH)-decontamination and characterization of culturable bacterial community from high-dose point HCH-contaminated soils. J. Appl. Microbiol. 106: 381-392.
  • Singh, A., Lal, R. 2009. Sphingobium ummariense sp. nov., a hexachlorocyclohexane (HCH)-degrading bacterium, isolated from HCH-contaminated soil. Int. J. Syst. Evol. Microbiol. 59: 162-166.
  • Raina, V., Rentsch, D., Geiger, T., Sharma, P., Buser, H. R., Holliger, C., Lal, R., Kohler, H. P. 2008. New metabolites in the degradation of alpha- and gamma-hexachlorocyclohexane (HCH): pentachlorocyclohexenes are hydroxylated to cyclohexenols and cyclohexenediols by the haloalkane dehalogenase LinB from Sphingobium indicum B90A. J. Agric. Food Chem. 56: 6594-6603.
  • Kumar, M., Verma, M., Lal, R. 2008. Devosia chinhatensis sp. nov., isolated from a hexachlorocyclohexane (HCH) dump site in India. Int. J. Syst. Evol. Microbiol. 58: 861-865.
  • Raina, V., Suar, M., Singh, A., Prakash, O., Dadhwal, M., Gupta, S. K., Dogra, C., Lawlor, K., Lal, S., van der Meer, J. R., Holliger, C., Lal, R. 2008. Enhanced biodegradation of hexachlorocyclohexane (HCH) in contaminated soils via inoculation with Sphingobium indicum B90A. Biodegradation. 19: 27-40.
  • Malhotra, S., Sharma, P., Kumari, H., Singh, A., Lal, R. 2007. Localization of HCH catabolic genes (lin genes) in Sphingobium indicum B90A. Indian J. Microbiol. 47: 271-275.

 

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